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Image Search Results
Journal: International Journal of Nanomedicine
Article Title: A novel method for synthesizing PEGylated chitosan nanoparticles: strategy, preparation, and in vitro analysis
doi: 10.2147/IJN.S17190
Figure Lengend Snippet: Gel retardation assay: lane 1) control 10 bp DNA ladder; lane 2 and 3 control siGLO, and lane 4 and 5 are PEGylated chitosan complexed siGLO at a ratio of 200:1 (w/w). In all groups the siGLO concentration was kept constant at 6 μg/mL. The disappearance of the band size at 40 bp in PEGylated chitosan complexed siGLO (lanes 4 and 5), compared to siGlO control (lanes 2 and 3), indicates the complete complexation of the siGLO.
Article Snippet: The PEGylated
Techniques: Electrophoretic Mobility Shift Assay, Control, Concentration Assay
Journal: International Journal of Nanomedicine
Article Title: A novel method for synthesizing PEGylated chitosan nanoparticles: strategy, preparation, and in vitro analysis
doi: 10.2147/IJN.S17190
Figure Lengend Snippet: Transfection efficiency of nanoparticles on Neuro2a cells after 4 hours of incubation at 37°C and 5% CO 2 : a ) PEGylated-chitosan-TPP:siGLO nanoparticles; b ) chitosan-TPP:siGLO nanoparticles; c ) siGLO only (negative control); d ) chitosan-TPP nanoparticles (negative control); e ) cells only (negative control) Abbreviation: TPP, sodium tripolyphosphate.
Article Snippet: The PEGylated
Techniques: Transfection, Incubation, Negative Control
Journal: International Journal of Nanomedicine
Article Title: A novel method for synthesizing PEGylated chitosan nanoparticles: strategy, preparation, and in vitro analysis
doi: 10.2147/IJN.S17190
Figure Lengend Snippet: Designed CS-TPP-PEG-siGLO nanoparticle cytotoxicity was investigated using Neuro2a cells. For the experiment, various control nanoparticle formulations were used, the Neuro2a cells were exposed for 4 hours and cell viability was evaluated using spectrophotometer at 490 nm using standard MTS assays (n = 3).
Article Snippet: The PEGylated
Techniques: Control, Spectrophotometry